Nā Papahana ʻenehana Peptide Synthesis
ʻO nā peptides lōʻihi (30 - 60 amino acids), nā peptides paʻakikī (lipopeptides, glycopeptides), cyclic peptides, non-natural amino acid peptides, peptide-nucleic acids, peptide-liʻiliʻi mole, peptide-proteins, peptide-radionuclides, etc.
Hoʻohui ʻia ʻo Solid-Phase Peptide Synthesis (SPPS)
ʻO ka wai-Phase Peptide Synthesis (LPPS)
ʻO ka hoʻoheheʻe ʻia ʻana o ka peptide Phase Liquid-Soild Phase Synthesis (L/SPPS)
Hoʻolālā Pūʻulu Hoʻopalekana liʻiliʻi no SPPS (MP-SPPS)
E hoʻomaʻamaʻa i ke kaʻina hana ma ka hōʻemi ʻana i ka hoʻohana ʻana i nā hui pale orthogonal i ka wā synthesis; e ho'ēmi i ke kumukūʻai o nā reagents pipiʻi (e like me Fmoc/tBu); pale i nā ʻaoʻao ʻaoʻao (e like me ka deprotection premature).
Ua waiho aku ka hui ma luna o 60 mau palapala noi, me ʻehā mau hōʻailona ma ka European Union a me ʻekolu ma ʻAmelika Hui Pū ʻIa, a ua loaʻa iā ia nā hoʻopaʻa inoa kope no nā hana ʻehā.
Nā Papahana Hoʻololi Peptide
Ma ka hoʻokomo ʻana i nā pūʻulu tracer (e like me nā hui fluorescent, biotin, radioisotopes) i loko o nā peptides, hiki ke hoʻokō ʻia nā hana e like me ka nānā ʻana, ʻike, a i ʻole ka hōʻoia ʻana.
Hoʻonui ka PEGylation i nā waiwai pharmacokinetic o nā peptides (e laʻa, hoʻonui i ka hapalua o ke ola a hōʻemi i ka immunogenicity).
Nā lawelawe Hoʻohui Peptide（P-Drug Conjugate）
ʻEkolu-element architecture o ka ʻōnaehana lāʻau i manaʻo ʻia:
Hoʻopili i ka Peptide: Hoʻopili kikoʻī i nā mea loaʻa / antigens ma ka ʻili o nā maʻi maʻi (e like me nā maʻi maʻi maʻi);
Linker: Hoʻopili i ka peptide a me ka lāʻau, hoʻoponopono i ka hoʻokuʻu ʻana i ka lāʻau (cleavable/non-cleavable design);
Uku Laau Laau: Hoʻopuka i nā cytotoxins a i ʻole nā mea lapaʻau (e like me nā lāʻau chemotherapeutic, radionuclides).
Nā Papahana ʻenehana Hoʻokumu Peptide
Nā Pūnaehana Hoʻouka Lapaʻau: Ke hoʻohana nei i nā ʻenehana hoʻopuka holomua e like me liposomes, polymeric micelles, a me nā nanoparticles.
ʻO ka ʻōnaehana hoʻopuka lāʻau hou e hoʻolōʻihi i ka lōʻihi o ka hoʻokuʻu ʻana i ka lāʻau lapaʻau vivo, e hiki ai i ka hoʻoponopono pinepine ʻana i ka dosing, a laila e hoʻonui ai i ka mālama ʻana i ka maʻi.
E hoʻohana i ka ʻenehana desalting pūnaewele 2D-LC e hoʻokō i ka ʻike kūpono o nā haumia paʻakikī. Hiki i kēia ʻenehana ke hoʻoponopono pono i ka pilikia hoʻohālikelike ma waena o ka buffer - loaʻa nā pae kelepona a me ka ʻike kikoʻī nui.
ʻO ka hoʻohui ʻana o ka Design of Experiments (DoE), ka nānā ʻana i nā ʻenehana, a me nā ʻenehana hoʻohālike helu e hoʻonui nui i ka pono o ka hoʻomohala ʻana i ke ʻano analytical a me ka paʻa o ka hopena.
Nā Mana Kiko
1. Hoʻohālikelike ʻano huahana
2. Hoʻomohala a me ka hōʻoia ʻana i ke ʻano Analytical
3.Stibil Study
4. Impurity Profiling Identification
JY FISTM Hoʻomaʻemaʻe ʻenehana Pūnaewele
1. Hoʻomau Chromatography
Hoʻohālikelike ʻia i ka chromatography batch, hāʻawi ia i nā pono o ka hoʻohana ʻana i ka solvent haʻahaʻa, ʻoi aku ka maikaʻi o ka hana ʻana, a me ka scalability kiʻekiʻe.
2. High-Performance Liquid Chromatography System1.
3.ʻO ka wikiwiki kaʻawale me ka hiki ke hoʻololi i nā peptides like ʻole
Mālama i ka peptide structural integrity a me ka bioactivity, maʻalahi i hana hou ʻia me ka wai.
ʻOi aku ka maikaʻi ma mua o ka lyophilization, me ka scalability wikiwiki i nā pae hana ʻoihana.
Hoʻohana nui ʻia ka Recrystallization i nā hoʻolālā Liquid-Phase Peptide Synthesis (LPPS) e kiʻi i nā peptides maʻemaʻe kiʻekiʻe a me nā ʻāpana ʻoiai e hoʻopaʻa pono ana i nā hale aniani, e hāʻawi ana i nā pono kūpono.
Nā Mana Kiko
1. Hoʻohālikelike ʻano huahana
2. Hoʻomohala a me ka hōʻoia ʻana i ke ʻano Analytical
3.Stibil Study
4. Impurity Profiling Identification
Nā Lako Keʻena Lab a me ka Pilot
LAB
ʻO ka Peptide Synthesizer piha
20-50 L Reactors
YXPPSTM
Prep-HPLC（DAC50 – DAC150）
Nā mea hoʻomaloʻo maloʻo (0.18 m2 – 0.5m2)
PILOT
3000L SPPS
500L-5000L LPPS
Prep-HPLC DAC150 - DAC 1200mm
Pūnaehana ʻOhi ʻakomi
Nā Mea Hoʻomaloʻo Paʻa
Pahu Mea maloo
